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In Vitro Nematocidal Activity of Plant Extracts of Mexican Flora against Haemonchus contortus Fourth Larval Stage
Author(s) -
Aguilar Hector Hugo Galicia,
De Gives Pedro Mendoza,
Sánchez David Osvaldo Salinas,
Arellano María Eugenia López,
Hernández Enrique Liébano,
Aroche Ulises López,
ValladaresCisneros Guadalupe
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.075
Subject(s) - haemonchus contortus , biology , larva , tagetes , in vitro , botany , traditional medicine , veterinary medicine , medicine , biochemistry
Fourteen plant extracts were evaluated in vitro against the fourth larval stage of Haemonchus contortus . The plants species used were Tagetes erecta, Argemone mexicana , and Castela tortuosa. The assays were run in 24‐well cell culture plates at room temperature with three replicates. After exposure, aliquots were taken from the corresponding wells and transferred to a microscope for observation. Evaluation criteria were based on the average of live and/or dead larvae. ANOVA test and Tukey test were used to determine significant differences among the treatments. After 96 h, the T. erecta acetonic extract produced 99.7% lethal activity, followed by C. tortuosa hexanic extract (85.8%) and T. erecta methanolic extract (58.3%) ( P < 0.0001).
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