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Flow Cytometry to Evaluate Anaplasma marginale Parasitemia Using a Fluorescent Nucleic Acid Stain
Author(s) -
Moretta Rosalía,
Ruybal Paula,
Mesplet María,
Petrigh Romina,
Nuñez Pablo,
Gil Gonzalo,
Wilkowsky Silvina,
Garbossa Graciela,
Farber Marisa
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.039
Subject(s) - flow cytometry , parasitemia , stain , nucleic acid , anaplasma , fluorescence , chemistry , microbiology and biotechnology , cytometry , biology , virology , staining , biochemistry , immunology , plasmodium falciparum , genetics , tick , physics , quantum mechanics , malaria
In this work we describe a flow cytometry–based method using SYTO16 (a DNA intercalating agent) to quantify Anaplasma marginale –infected erythrocytes in blood from bovine animals. The linearity and reproducibility of the results obtained with SYTO16 labeling followed by flow cytometry analysis make it a suitable approach for measurement of parasitemia in A. marginale infections.