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Characterization of a Vitellogenin Gene Fragment in Boophilus microplus Ticks
Author(s) -
GranjenoColin Gabriela,
HernandezOrtiz Ruben,
Mosqueda Juan,
EstradaMondaca Sandino,
Figueroa Julio V.,
GarciaVazquez Zeferino
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.034
Subject(s) - biology , gene , nucleic acid sequence , homology (biology) , vitellogenin , microbiology and biotechnology , peptide sequence , complementary dna , genetics , amino acid , sequence analysis
The objective of this study was to isolate, clone, and characterize a fragment of the vitellogenin (Vg) gene from a B. microplus tick strain from Mexico. Using cDNA and specific primers, an 1800‐bp fragment was amplified, cloned, and transformed in into E. coli , and then sequenced. Comparative analysis with a previously reported sequence showed 99% identity at both the nucleotide and amino acid level. The predicted amino acid sequence of the Mexican Vg has 6 positive mutations. There is an insertion of an aspartic acid on position 26 and a deletion on position 552 with respect to the reported sequence. There were 11 predicted glycosylation sites conserved in both strains. It is concluded that there is a high sequence homology of Vg in both strains.