Premium
Secreted Proteins of Avibacterium paragallinarum Are Lethal for Chicken Embryo
Author(s) -
PérezMárquez Víctor,
PérezMéndez Alma,
IbarraCaballero Jorge,
GómezLugo Gabriela,
VázquezCruz Candelario,
Vaca Sergio,
NegreteAbascal Erasmo
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.007
Subject(s) - microbiology and biotechnology , actinobacillus pleuropneumoniae , embryo , biology , bacteria , antibody , blot , inoculation , yolk , serotype , gene , immunology , biochemistry , ecology , genetics
Avibacterium paragallinarum causes infectious coryza in chickens. This bacterium secretes proteins of 110 kDa (a putative RTX protein) and 120 kDa. Expression of these proteins increases by the addition of CaCl 2 , MgSO 4 , MnSO 4 , or ferric ammonium citrate and diminishes with CuSO 4 or ZnCl 2 . Protein expression is optimal at 37°C and pH 7.5. Mortality (90–100%) of chicken embryos was observed when secreted proteins (SPs) from A. paragallinarum reference or field isolates (serogroup A or C) were inoculated via yolk sac and was not observed when SPs from A. avium , a chicken respiratory tract indigenous bacterium, were inoculated. A. paragallinarum SPs could contain toxins responsible for the embryo deaths. Indeed, presence of the putative RTX protein of 110 kDa was confirmed by Western blotting with antibodies against the Actinobacillus pleuropneumoniae RTX ApxI, a closely related RTX protein.