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Localization of Entamoeba histolytica Amebopore in Amebic Liver Abscesses in Hamsters
Author(s) -
González A.,
Monterrubio D.,
Nequiz M.,
López R.,
Olivos A.,
De Léon C. García,
Tello E.,
Salaiza N.,
Ramos E.,
Gudiño M.,
Montfort I.,
PérezTamayo R.
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1428.006
Subject(s) - polyclonal antibodies , entamoeba histolytica , western blot , hamster , in vivo , antibody , microbiology and biotechnology , extracellular , antiserum , chemistry , hepatocyte , biology , biochemistry , in vitro , immunology , gene
Amebopore was purified from axenically grown trophozoites of the Entamoeba histolytica strain HM1:IMSS. The purification procedure involved Mono Q anion‐exchange chromatography and electroelution. Sequence analysis of the final product revealed that amebopore A was completely pure. Polyclonal antibodies against the purified amebopore were obtained from rabbits, and Western blot studies demonstrated their specificity. Sections of experimental, acute (1, 2, 3, and 4 days), amebic liver abscesses produced in hamsters were stained with the anti‐amebopore antibody; in all the analyzed stages, amebopore appeared as a constitutively expressed cytoplasmic molecule in trophozoites. No extracellular or hepatocyte‐membrane amebopore was found. This study is the first to trace amebopore in an in vivo model of amebic liver abscesses.