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Anaerobic Respiration in Engineered Escherichia coli with an Internal Electron Acceptor to Produce Fuel Ethanol
Author(s) -
Peterson Joy Doran,
Ingram Lonnie O.
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1419.020
Subject(s) - biomass (ecology) , chemistry , lignocellulosic biomass , ethanol , ethanol fuel , metabolic engineering , biofuel , escherichia coli , biochemistry , biochemical engineering , enzyme , fermentation , food science , microbiology and biotechnology , biology , gene , agronomy , engineering
Environmental concerns and unease with U.S. dependence on foreign oil have renewed interest in converting biomass into fuel ethanol. The volume of plant matter available makes lignocellulose conversion to ethanol desirable, although no one isolated organism has been shown to break bonds in lignocellulose and efficiently metabolize resulting sugars into one product. This work reviews directed engineering coupled with metabolic evolution resulting in microbial biocatalysts that produce up to 45 g L −1 ethanol in 48 hours in a simple mineral salts medium and that convert various compounds of lignocellulosic materials to ethanol. Mutations contributing to ethanologenesis are discussed along with adding enzymatic capabilities to existing biocatalysts in order to decrease the commercial enzymes required to reduce plant matter into fermentable sugars.