Premium
Determination of Thymosin β 4 and Protein in Human Wound Fluid after Abdominal Surgery
Author(s) -
BODENDORF S.,
BORN G.,
HANNAPPEL E.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1415.023
Subject(s) - thymosin , abdominal surgery , chemistry , wound healing , abdominal wound , surgery , medicine , chromatography , biochemistry
: Wound fluids were collected up to 60 h after abdominal surgery. Immediately after obtaining the wound fluid by Robinson drainage, wound fluid was centrifuged to remove blood cells and inflammatory cells. The concentration of total protein as well as of thymosin β 4 was determined in the cell‐free supernatant solution. Total protein concentration decreased from about 50 g/L to 30 g/L within 60 h after surgery. After surgery we observed a concentration of up to 20 mg thymosin β 4 per liter decreasing to about 1 mg/L with time. Neither thymosin β 10 nor oxidized thymosin β 4 was detected in human wound fluid.