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Corticotropin‐releasing Hormone Receptor Subtypes in the Rat Anterior Pituitary after Two Types of Restraint Stress
Author(s) -
Klenerova Vera,
Sery Omar,
Hynie Sixtus
Publication year - 2008
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1410.043
Subject(s) - corticotropin releasing hormone , medicine , endocrinology , anterior pituitary , receptor , hormone , glyceraldehyde 3 phosphate dehydrogenase , messenger rna , stressor , chemistry , biology , gene , neuroscience , biochemistry
The stress response in anterior pituitary (AP) is mediated by corticotropin‐releasing hormone (CRH) acting through CRH‐R1 and ‐R2, however, the function of CRH‐R2 in AP is still not fully elucidated. We used 1‐h long restraint (IMO) as well as restraint combined with water immersion (IMO+C). Using real‐time PCR we quantified mRNA expression of CRH‐R1, CRH‐R2α‐soluble and ‐insoluble, and cAMP response element binding (CREB), with reference gene GAPDH. In control AP, CRH‐R1 mRNA was up to 20‐fold higher than levels of CRH‐R2α‐soluble or CRH‐R2α‐insoluble mRNA. IMO reduced CRH‐R1 mRNA to 47% and 63% of control levels 1 and 2 h after the onset of stressor, respectively, while IMO+C did not produce significant changes. Our data demonstrated that these stressors did not change CRH‐R2α mRNA, unlike the very significant response of CRH‐R1 to IMO.