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Synovial Fluid Fibroblasts and Lymphocytes Support the Osteoclastogenesis in Human Psoriatic Arthritis
Author(s) -
MORI GIORGIO,
CANTATORE FRANCESCO P.,
BRUNETTI GIACOMINA,
ORANGER ANGELA,
COLAIANNI GRAZIANA,
QUARTA LAURA,
CORRADO ADDOLORATA,
COLUCCI SILVIA,
GRANO MARIA
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1402.013
Subject(s) - rankl , peripheral blood mononuclear cell , psoriatic arthritis , synovial fluid , bone resorption , fibroblast , tumor necrosis factor alpha , osteoclast , chemistry , psoriasis , immunology , medicine , cancer research , in vitro , pathology , receptor , activator (genetics) , osteoarthritis , biochemistry , alternative medicine
: Aggressive bone resorption is well recognized in psoriatic arthritis (PsA). By using in vitro osteoclastogenesis models, consisting of unstimulated mononuclear cells from peripheral blood (PBMCs) and synovial fluid (SFMCs) of PsA patients, we demonstrated the spontaneous OC formation in both culture systems. In PBMCs the osteoclastogenesis was T cell‐dependent while it was T cell‐ and fibroblast‐dependent in SFMCs. T cells isolated from PBMCs and SFMCs of PsA patients overexpressed RANKL and TNF‐α, and fibroblasts from SFMCs overexpressed RANKL. Thus, T cells and fibroblasts from synovial fluid through the production of osteoclastogenic cytokines could support the OC formation in PsA patients.