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Osteoblasts Cultured on Three‐Dimensional Synthetic Hydroxyapatite Implanted on a Chick Allantochorial Membrane Induce Ectopic Bone Marrow Differentiation
Author(s) -
MANCINI LUCIA,
TAMMA ROBERTO,
SETTANNI MARIA,
CAMERINO CLAUDIA,
PATANO NICOLA,
GRECO GIOVANNI,
STRIPPOLI MAURIZIO,
ZALLONE ALBERTA
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1402.008
Subject(s) - bone marrow , haematopoiesis , microbiology and biotechnology , chemistry , extracellular matrix , matrix (chemical analysis) , biomaterial , osteoblast , implant , membrane , anatomy , biology , stem cell , immunology , in vitro , medicine , biochemistry , surgery , organic chemistry , chromatography
: Osteoblast (OB) activities have been studied on hydroxyapatite three‐dimensional (3D) scaffolds in comparison with traditional planar substrata. OBs cultured on 3D displayed increased proliferation, differentiation, and matrix protein synthesis, when compared to 2D cultures on the same substrata. Confluent cultures, however, could not be maintained for long, due to insufficient fluid diffusion within 3D scaffolds that impaired cell viability. Thus, confluent OB 3D cultures were implanted on the allantochorial membrane of chick embryos. Vessels from the embryo colonized the bone‐like network giving rise in the presence of OBs to an ectopic bone marrow formation in the intratrabecular spaces. In the absence of OBs, when the biomaterial alone was implanted, blood vessels were still present but hematopoietic marrow was absent. In both cases osteoclasts (OCs) derived from the host were found on the implant surface. These results indicated that scaffolds with cells can be easily vascularized and confirmed the role of OBs in the definition of the microenvironment that induce blood marrow differentiation in the intratrabecular spaces.