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Identification of Histidine‐Rich Glycoprotein, a Potential Autoantigen, in Human and Rat Brain Preparations
Author(s) -
BALLROSEN CATRIN,
PRIMAKRUBINOVICZ HAGIT,
KORCZYN AMOS D.,
WANG NINGSHAN,
FARCHI ADEL,
SHAVIT SARA,
SHOENFELD YEHUDA,
CHAPMAN JOAB
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1398.053
Subject(s) - glycoprotein , human brain , biology , homology (biology) , western blot , microbiology and biotechnology , biochemistry , gene , neuroscience
: β 2 ‐glycoprotein‐I (β 2 ‐GPI, also known as apolipoprotein H) is a major autoantigen in the antiphospholipid syndrome (APS), a disease commonly affecting the central nervous system. We examined whether β2‐GPI and similar proteins exist in rat and human brains. No expression was found on Northern blot analysis of human brain. Utilizing a standard procedure for the isolation of serum β2‐GPI we purified a 100 kD human brain protein, which was found by peptide sequencing to have full homology with the serum protein, histidine‐rich glycoprotein (HRGP). Expression of HRGP in rat and human brain was established by RT‐PCR studies and a partial sequence of rat brain HRGP was obtained showing 68% homology with the human protein. IgG from most APS patients bound to HRGP, which shares distinct biochemical properties with β2‐GPI, is present in the brain and may be an important autoantigen.