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Involvement of AMPK Signaling Cascade in Capsaicin‐Induced Apoptosis of HT‐29 Colon Cancer Cells
Author(s) -
KIM YOUNG MIN,
HWANG JINTAEK,
KWAK DONG WOOK,
LEE YUN KYUNG,
PARK OCK JIN
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1397.053
Subject(s) - ampk , protein kinase a , apoptosis , capsaicin , chemistry , amp activated protein kinase , cancer research , kinase , medicine , endocrinology , microbiology and biotechnology , biology , receptor , biochemistry
: Adenosine monophosphate (AMP)‐activated protein kinase (AMPK) is activated during ATP‐depleting metabolic states, such as hypoxia, heat shock, oxidative stress, and exercise. As a highly conserved heterotrimeric kinase that functions as a major metabolic switch to maintain energy homeostasis, AMPK has been shown to exert as an intrinsic regulator of mammalian cell cycle. Moreover, AMPK cascade has emerged as an important pathway implicated in cancer control. In this article, we have investigated the effects of capsaicin on apoptosis in relation to AMPK activation in colon cancer cell.Capsaicin‐induced apoptosis was revealed by the presence of nucleobodies in the capsaicin‐treated HT‐29 colon cancer cells. Concomitantly, the activation of AMPK and the increased expression of the inactive form of acetyl‐CoA carboxylase (ACC) were detected in capsaicin‐treated colon cancer cells. We showed that both capsaicin and 5′‐aminoimidazole‐4‐carboxamide‐1‐β‐D‐ribonucleoside (AICAR), an AMPK activator possess the AMPK‐ activating capacity as well as apoptosis‐inducing properties.Evidence of the association between AMPK activation and the increased apoptosis in HT‐29 colon cancer cells by capsaicin treatment, and further findings of the correlation of the activated AMPK and the elevated apoptosis by cotreatment of AICAR and capsaicin support AMPK as an important component of apoptosis, as well as a possible target of cancer control.