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The Effect of Resveratrol on a Cell Model of Human Aging
Author(s) -
STEFANI MAURIZIO,
MARKUS M. ANDREA,
LIN RUBY C. Y.,
PINESE MARK,
DAWES IAN W.,
MORRIS BRIAN J.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1396.001
Subject(s) - resveratrol , sirtuin , sirtuin 1 , microbiology and biotechnology , transcription factor , senescence , biology , microarray analysis techniques , cell cycle , cell , gene , chemistry , gene expression , genetics , downregulation and upregulation , biochemistry , acetylation
: The natural polyphenol resveratrol stimulates sirtuins and extends lifespan. Here resveratrol inhibited expression of replicative senescence marker INK4a in human dermal fibroblasts, and 47 of 19,000 genes from microarray experiments were differentially expressed. These included genes for growth, cell division, cell signaling, apoptosis, and transcription. Genes involved in Ras and ubiquitin pathways, Ras‐GRF1 , RAC3 , and UBE2D3 , were downregulated. The changes suggest resveratrol might alter sirtuin‐regulated downstream pathways, rather than sirtuin activity. Serum deprivation and high confluency caused nuclear translocation of the SIRT1‐regulated transcription factor FOXO3a. Our data indicate resveratrol's actions might cause FOXO recruitment to the nucleus.