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Glyoxal‐Induced Premature Senescence in Human Fibroblasts
Author(s) -
SEJERSEN HENRIK,
RATTAN SURESH I. S.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1395.057
Subject(s) - hormesis , senescence , glyoxal , microbiology and biotechnology , apoptosis , cell division , dna damage , telomere , cell , chemistry , autophagy , premature aging , fibroblast , cell growth , programmed cell death , biology , cancer research , dna , biochemistry , oxidative stress , genetics , in vitro , organic chemistry
: Mild stress‐induced hormesis is an effective strategy to intervene in the aging process. Repeated exposure of human skin fibroblasts to 41°C heat shock for 1 h twice a week is an example of mild stress that has many hormetic effects, including improved resistance to other stressors. We are now developing an experimental model system of sugar‐induced premature senescence, which can be useful to test the hormetic and antiaging effects of other stresses. Our present studies show that early‐passage human skin fibroblasts treated with 1 mM glyoxal for 72 h undergo premature senescence in terms of enlarged cell size, inhibition of cell division, slowing down of cell growth, a decrease in the number of DNA synthesizing cells, and increased resistance to apoptosis.