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Quantum Dot‐Based Mortalin Staining as a Visual Assay for Detection of Induced Senescence in Cancer Cells
Author(s) -
KAUL ZEENIA,
YAGUCHI TOMOKO,
CHIURA HIROSHI X.,
KAUL SUNIL C.,
WADHWA RENU
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1395.040
Subject(s) - staining , fluorescence , senescence , quantum dot , chemistry , cell , viability assay , resolution (logic) , biophysics , in vitro , cancer cell , excitation , dapi , microbiology and biotechnology , nanotechnology , cancer , materials science , apoptosis , biology , biochemistry , pathology , optics , medicine , computer science , physics , genetics , artificial intelligence , quantum mechanics
: Quantum dots (QDs) are fluorescent nanocrystals that are emerging as fine alternatives to the conventional organic dyes. They have several advantages including greater photostability and a wider range of excitation–emission wavelengths. By using mortalin staining as a model, we initially demonstrated that the QDs are more stable and provide better resolution in protein imaging in fixed cells. With the help of an internalizing antibody, we generated internalizing QD (i‐QD) and demonstrated its inertness to cell replication, structure, and viability. Based on the superior resolution, stability and inertness, we propose the use of QD staining of mortalin as a cell‐based visual assay to screen for senescence‐inducing drugs, proteins, and siRNAs.