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Knocking Down p53 with siRNA Does Not Affect the Overexpression of p21 WAF‐1 after Exposure of IMR‐90 hTERT Fibroblasts to a Sublethal Concentration of H 2 O 2 Leading to Premature Senescence
Author(s) -
ZDANOV STEPHANIE,
DEBACQCHAINIAUX FLORENCE,
TOUSSAINT OLIVIER
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1395.034
Subject(s) - senescence , telomerase , telomerase reverse transcriptase , dna damage , microbiology and biotechnology , cell cycle , cell , small interfering rna , telomere , biology , cell cycle checkpoint , chemistry , cancer research , cell culture , transfection , dna , genetics , gene
: Premature senescence of IMR‐90 human diploid fibroblasts (HDFs) expressing telomerase was induced by exposure to sublethal concentration of H 2 O 2 , with appearance of several biomarkers of cellular senescence like enlarged cell shape, senescence‐associated β‐galactosidase (SA ß‐gal) activity, and cell cycle arrest. The induction of stress‐induced premature senescence (SIPS) was associated with a transient increase in DNA‐binding activity of p53 and an increased expression of p21 WAF‐1 . p53 small interferent RNA (siRNA) affected the basal level of p21 WAF‐1 mRNA but did not affect the overexpression of p21 WAF‐1 after stress. This siRNA approach confirms previous results obtained with other methods.