Premium
Development of New‐Generation HU‐PBMC‐NOD/SCID Mice to Study Human Islet Alloreactivity
Author(s) -
KING MARIE,
PEARSON TODD,
SHULTZ LEONARD D.,
LEIF JEAN,
BOTTINO RITA,
TRUCCO MASSIMO,
ATKINSON MARK,
WASSERFALL CLIVE,
HEROLD KEVAN,
MORDES JOHN P.,
ROSSINI ALDO A.,
GREINER DALE L.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1394.011
Subject(s) - nod , humanized mouse , immunology , nod mice , peripheral blood mononuclear cell , immune system , biology , autoimmunity , gene , in vitro , genetics
: The use of “humanized” mice represents an appealing translational model for studies of the pathogenesis of immune‐mediated diseases and for the evaluation of potential therapeutics. The utility of humanized mice depends on their ability to model the human immune system with high fidelity, and, in this respect, previous models have fallen short. The recently developed NOD‐ scid Il2rγ null mouse, however, exhibits greatly enhanced ability to support the engraftment of human peripheral blood mononuclear cells. Herein, we describe the challenges of recapitulating human immunity in humanized mice and features of NOD‐ scid Il2rγ null mice that help overcome them.