Premium
Examining Ca 2+ Extrusion of Na + /Ca 2+ ‐K + Exchangers
Author(s) -
ALTIMIMI HAIDER F.,
SCHNETKAMP PAUL P. M.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1387.056
Subject(s) - chemistry , extrusion , intracellular , ion exchange , ionic bonding , ion transporter , analytical chemistry (journal) , ion , membrane , biophysics , inorganic chemistry , chromatography , biochemistry , materials science , organic chemistry , biology , metallurgy
Na + /Ca 2+ ‐K + exchangers (NCKX) are plasma membrane transporters that are thought to mainly mediate Ca 2+ extrusion (along with K + ) at the expense of the Na + electrochemical gradient. However, because they are bidirectional, most assays have relied on measuring their activity in the reverse (Ca 2+ import) mode. Herein we describe a method to control intracellular ionic conditions, and examine the forward (Ca 2+ extrusion) mode of exchange of NCKX2.