The Na + /Ca 2+  Exchanger Isoform 3 (NCX3) but Not Isoform 2 (NCX2) and 1 (NCX1) Singly Transfected in BHK Cells Plays a Protective Role in a Model of  in Vitro  Hypoxia | Zendy

SECONDO AGNESE | Zendy; STAIANO ILARIA ROSARIA | Zendy; SCORZIELLO ANTONELLA | Zendy; SIRABELLA ROSSANA | Zendy; BOSCIA FRANCESCA | Zendy; ADORNETTO ANNAGRAZIA | Zendy; CANZONIERO LORELLA MARIA TERESA | Zendy; DI RENZO GIANFRANCO | Zendy; ANNUNZIATO LUCIO | Zendy

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The Na + /Ca 2+ Exchanger Isoform 3 (NCX3) but Not Isoform 2 (NCX2) and 1 (NCX1) Singly Transfected in BHK Cells Plays a Protective Role in a Model of in Vitro Hypoxia

Author(s) -

SECONDO AGNESE,

STAIANO ILARIA ROSARIA,

SCORZIELLO ANTONELLA,

SIRABELLA ROSSANA,

BOSCIA FRANCESCA,

ADORNETTO ANNAGRAZIA,

CANZONIERO LORELLA MARIA TERESA,

DI RENZO GIANFRANCO,

ANNUNZIATO LUCIO

Publication year - 2007

Publication title -

annals of the new york academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.712

H-Index - 248

eISSN - 1749-6632

pISSN - 0077-8923

DOI - 10.1196/annals.1387.052

Subject(s) - transfection , gene isoform , intracellular , chemistry , cell culture , hypoxia (environmental) , microbiology and biotechnology , calcium , biochemistry , biology , gene , oxygen , genetics , organic chemistry

Chemical hypoxia produces depletion of ATP, intracellular Ca 2+ overload, and cell death. The role of Na + /Ca 2+ exchanger (NCX), the major plasma membrane Ca 2+ extruding system, has been explored in chemical hypoxia using BHK cells stably transfected with the three mammalian NCX isoforms: NCX1, NCX2, and NCX3. Here we report that the three isoforms show similar activity evaluated as [Ca 2+ ] i increase evoked by Na + ‐free medium exposure in Fura‐2‐loaded single cells and NCX3 transfected cells are less vulnerable to chemical hypoxia compared to NCX1‐ and NCX2‐transfected cells, suggesting that NCX3 could play a more relevant protective role during chemical hypoxia.

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