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Analysis of Calcium Changes in Endoplasmic Reticulum during Apoptosis by the Fluorescent Indicator Chlortetracycline
Author(s) -
CERELLA CLAUDIA,
MEARELLI CRISTINA,
DE NICOLA MILENA,
D'ALESSIO MARIA,
MAGRINI ANDREA,
BERGAMASCHI ANTONIO,
GHIBELLI LINA
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1387.021
Subject(s) - endoplasmic reticulum , thapsigargin , apoptosis , fluorescence , microbiology and biotechnology , calcium , cytosol , chemistry , biophysics , biochemistry , biology , enzyme , physics , organic chemistry , quantum mechanics
Many studies suggest that endoplasmic reticulum (ER) Ca 2+ pool rather than cytosolic Ca 2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca 2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca 2+ depletor, confirm the findings that apoptotic cells have a Ca 2+ ‐depleted ER, in contrast with treated but still viable cells.