Analysis of Calcium Changes in Endoplasmic Reticulum during Apoptosis by the Fluorescent Indicator Chlortetracycline | Zendy

CERELLA CLAUDIA | Zendy; MEARELLI CRISTINA | Zendy; DE NICOLA MILENA | Zendy; D'ALESSIO MARIA | Zendy; MAGRINI ANDREA | Zendy; BERGAMASCHI ANTONIO | Zendy; GHIBELLI LINA | Zendy

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Analysis of Calcium Changes in Endoplasmic Reticulum during Apoptosis by the Fluorescent Indicator Chlortetracycline

Author(s) -

CERELLA CLAUDIA,

MEARELLI CRISTINA,

DE NICOLA MILENA,

D'ALESSIO MARIA,

MAGRINI ANDREA,

BERGAMASCHI ANTONIO,

GHIBELLI LINA

Publication year - 2007

Publication title -

annals of the new york academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.712

H-Index - 248

eISSN - 1749-6632

pISSN - 0077-8923

DOI - 10.1196/annals.1387.021

Subject(s) - endoplasmic reticulum , thapsigargin , apoptosis , fluorescence , microbiology and biotechnology , calcium , cytosol , chemistry , biophysics , biochemistry , biology , enzyme , physics , organic chemistry , quantum mechanics

Many studies suggest that endoplasmic reticulum (ER) Ca 2+ pool rather than cytosolic Ca 2+ may play a crucial role in triggering apoptosis. In this study, we performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca 2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca 2+ depletor, confirm the findings that apoptotic cells have a Ca 2+ ‐depleted ER, in contrast with treated but still viable cells.

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