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Rapid Downregulation of NCX and PMCA in Hippocampal Neurons Following H 2 O 2 Oxidative Stress
Author(s) -
KIP SERTAC N.,
STREHLER EMANUEL E.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1387.005
Subject(s) - plasma membrane ca2+ atpase , oxidative stress , downregulation and upregulation , hippocampal formation , microbiology and biotechnology , chemistry , sodium calcium exchanger , homeostasis , calcium signaling , intracellular , internalization , biochemistry , biology , endocrinology , cell , atpase , gene , enzyme
Na + /Ca 2+ exchangers (NCXs) and plasma membrane Ca 2+ pumps (PMCAs) are crucial for intracellular Ca 2+ homeostasis and Ca 2+ signaling. Elevated [Ca 2+ ] i is a hallmark of neurodegenerative disease and stroke. Here we studied the short‐term effect of oxidative stress on the plasma membrane Ca 2+ extrusion systems in hippocampal neurons (HN) and found that after 2–3 h exposure to 300 μM H 2 O 2 , all NCXs and PMCAs were significantly downregulated at the RNA (NCX) and protein (PMCA) level. Rapid internalization and aggregation of the PMCA was also observed. Our data show that the plasma membrane calcium extrusion systems are sensitive early targets of neurotoxic oxidative stress.