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Anti‐inflammatory and Neuroprotective Effect of a Phytoestrogen Compound on Rat Microglia
Author(s) -
MAROTTA F.,
MAO G.S.,
LIU T.,
CHUI D.H.,
LORENZETTI A.,
XIAO Y.,
MARANDOLA P.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1386.033
Subject(s) - neuroprotection , microglia , pharmacology , chemistry , traditional medicine , inflammation , neuroscience , medicine , biology
Ovariectomized Wistar rats received orally 15 mg/kg of a phytoestrogen compound (genistein, daidzein, glycitein, black cohosh, angelica sin., licorice, vitex agnus) for 2 weeks to test its ability to modulate inflammatory microglia response. Microglial proliferation was tested by trypan blue and by absorbance. Serial supernatant sampling was performed for 24 h to check TNF‐α, IL‐β, IL‐6, and TGF‐β. LPS caused a time course increase of all cytokines, with IL‐β and TNF‐α peaking at the 12th hour, whereas IL‐6 and TGF‐β peaked at the 24 h observation. Rats fed with the phytoestrogen displayed a significantly lower level of proinflammatory cytokines and a higher level of TGF‐β, as shown also by Western blot analysis. This finding may offer promise in the field of nutraceutical intervention.