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A Microfluidic System for Saliva‐Based Detection of Infectious Diseases
Author(s) -
CHEN ZONGYUAN,
MAUK MICHAEL G.,
WANG JING,
ABRAMS WILLIAM R.,
CORSTJENS PAUL L. A. M.,
NIEDBALA R. SAM,
MALAMUD DANIEL,
BAU HAIM H.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1384.024
Subject(s) - microfluidics , fluidics , microfluidic chip , lab on a chip , saliva , lysis , cartridge , materials science , chip , chromatography , nanotechnology , chemistry , biology , computer science , microbiology and biotechnology , engineering , telecommunications , biochemistry , aerospace engineering , metallurgy
: A “lab‐on‐a‐chip” system for detecting bacterial pathogens in oral fluid samples is described. The system comprises: (1) an oral fluid sample collector; (2) a disposable, plastic microfluidic cassette (“chip”) for sample processing including immunochromatographic assay with a nitrocellulose lateral flow strip; (3) a platform that controls the cassette operation by providing metered quantities of reagents, temperature regulation, valve actuation; and (4) a laser scanner to interrogate the lateral flow strip. The microfluidic chip hosts a fluidic network for cell lysis, nucleic acid extraction and isolation, PCR, and labeling of the PCR product with bioconjugated, upconverting phosphor particles for detection on the lateral flow strip.