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Rapid Assay Format for Multiplex Detection of Humoral Immune Responses to Infectious Disease Pathogens (HIV, HCV, and TB)
Author(s) -
CORSTJENS PAUL L. A. M.,
CHEN ZONGYUANG,
ZUIDERWIJK MICHEL,
BAU HAIM H.,
ABRAMS WILLIAM R.,
MALAMUD DANIEL,
SAM NIEDBALA R.,
TANKE HANS J.
Publication year - 2007
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1384.016
Subject(s) - multiplex , virology , hepatitis c virus , antibody , human immunodeficiency virus (hiv) , immune system , multiplex polymerase chain reaction , virus , immunology , medicine , biology , polymerase chain reaction , bioinformatics , biochemistry , gene
: A novel assay is described for multiplex detection of antibodies against different pathogens from a single sample. The assay employs a modified lateral flow format (consecutive flow, CF) together with a sensitive reporter particle technology (up‐converting phosphor technology, UPT) that allows for fully instrumented assay analysis. Lateral flow (LF) strips developed for the detection of human antibodies against human immunodeficiency virus type‐1 and ‐2 (HIV‐1 and ‐2) with additional capture zones to detect antibodies against Myobacterium tuberculosis (TB) and hepatitis C Virus (HCV) provided the strips to test multiplexing. Data are presented that show the performance of the TB and HCV test, as well as two multiplex assays, TB with HIV and HCV with HIV.The TB/HCV assays demonstrate excellent detection capability, and HIV multiplexing does not affect the qualitative test result. The bench‐top CF format was converted to a microfluidic platform and a first prototype semiautomated chip capable of performing CF is presented here.