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Determination of Oxidative Stress Status and Concentration of TGF‐β1 in the Blood and Saliva of Osteoporotic Subjects
Author(s) -
YOUSEFZADEH GHOLAMREZA,
LARIJANI BAGHER,
MOHAMMADIRAD AZADEH,
HESHMAT RAMIN,
DEHGHAN GHOLAMREZA,
RAHIMI ROJA,
ABDOLLAHI MOHAMMAD
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1378.062
Subject(s) - tbars , saliva , oxidative stress , lipid peroxidation , medicine , thiobarbituric acid , endocrinology , osteoporosis , antioxidant , chemistry , biochemistry
Preliminary reports indicate the influence of oxidative stress and interleukins, particularly TGF‐β1, in maintenance of bone mass. This study was designed to determine any possible variations of cellular lipid peroxidation, the total antioxidant power, and concentration of TGF‐β1 in blood and saliva of osteoporotic subjects in comparison to healthy people. Blood and saliva samples of 22 osteoporotic women and 22 age‐matched healthy women were collected. Samples were analyzed for thiobarbituric acid‐reactive substances (TBARS) as a marker of lipid peroxidation, ferric reducing ability (total antioxidant power, TAP), and concentration of TGF‐β1. The blood and saliva TAP (mean ± SD) of osteoporotic subjects was significantly lower than that of healthy controls (606.65 ± 119.13 vs. 665.64 ± 63.73 mmol/L and 560.43 ± 84.70 vs. 612.05 ± 81.5, respectively). Blood and saliva TBARS (mean ± SD) of osteoporotic subjects were significantly higher than those of healthy controls (0.30 ± 0.04 vs. 0.26 ± 0.04 and 0.23 ± 0.03 vs. 0.16 ± 0.04 μmol/L, respectively). Concentrations of TGF‐β1 (mean ± SD) in plasma and saliva of osteoporotic subjects were not different in comparison to healthy subjects. Results indicate that persons with osteoporosis have an increased oxidative stress that is not accompanied by changes in TGF‐β1 levels. Use of supplementary antioxidants in osteoporotic patients may be helpful.