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The Direct and Indirect Effects of Serofendic Acid on Neuroprotection
Author(s) -
DOI YUKIKO,
LIANG JIANFENG,
KUNO REIKO,
ZANG GUIQIN,
KAWANOKUCHI JUN,
YAWATA IZUMI,
TAKEUCHI HIDEYUKI,
MIZUNO TETSUYA,
SUZUMURA AKIO
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1377.009
Subject(s) - neuroprotection , microglia , neurotrophin , glutamate receptor , neurotrophic factors , tumor necrosis factor alpha , pharmacology , cytokine , toxicity , chemistry , biology , inflammation , biochemistry , medicine , immunology , receptor
Serofendic acid is a novel neuroprotective factor isolated from fetal calf serum. To elucidate the mechanisms how serofendic acid exerts neuroprotection, we examined its effects on glutamate‐induced excito‐toxicity in mouse cortical neurons. The effects of serofendic acid on inflammatory cytokine and neurotrophin production by glial cells were also examined to evaluate the indirect neuroprotection. Serofendic acid significantly and dose dependently increased survival of mouse cortical neurons after 10 μM N‐methyl‐D‐asparate (NMDA) exposure. However, it did not affect production of inflammatory cytokines and neurotrophins by microglia as assessed by reverse transciption polymerase chain reaction (RT‐PCR) for mRNA expression and ELISA for protein levels, though it suppressed tumor necrosis factor (TNF)‐α production by astrocytes. Thus, serofendic acid works directly on neurons to protect against glutamate toxicity. Suppression of TNF‐α production by astoryctes may also synergistically exert neuroprotective functions of serofendic acid. Serofendic acid may be of use for the future therapeutic strategy against ischemic and degenerative neurological disorders.