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Association of SUMO4 M55V Polymorphism with Autoimmune Diabetes in Latvian Patients
Author(s) -
SEDIMBI SAIKIRAN K.,
SHASTRY ARUN,
PARK YONGSOO,
RUMBA INGRIDA,
SANJEEVI CARANI B.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1375.041
Subject(s) - type 1 diabetes , allele , diabetes mellitus , autoimmune diabetes , genetics , genetic predisposition , restriction fragment length polymorphism , polymorphism (computer science) , genotype , medicine , biology , gene , endocrinology
Small ubiquitin‐related modifier (SUMO4), located in IDDM5, has been identified as a potential susceptibility gene for type 1 diabetes mellitus (T1DM). The novel polymorphism M55V, causing an amino acid change in the evolutionarily conserved met55 residue has been shown to activate the nuclear factor κB (NF‐κB), hence the suspected role of SUMO4 in the pathogenicity of T1DM. The M55V polymorphism has been shown to be associated with susceptibility to T1DM in Asians, but not in Caucasians. Latent autoimmune diabetes in adults (LADA) is a slowly progressive form of T1DM and SUMO4 M55V has not been studied in LADA to date. The current study aims to test whether Latvians are similar to Caucasians in susceptibility to autoimmune diabetes (T1DM and LADA), with respect to SUMO4 M55V. We studied, age‐ and sex‐matched, Latvian T1DM patients ( n = 100) and healthy controls ( n = 90) and LADA patients ( n = 45) and healthy controls ( n = 95). SUMO4 M55V polymorphism was analyzed using polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP). The allelic frequencies of the A and G alleles were compared with HLA DR3–DR4‐positive and HLA DR3‐DR4‐negative patients to identify any potential relation between HLA DR3–DR4 and SUMO4 M55V. We found no significant association between SUMO4 M55V and T1DM susceptibility in Latvians, the results being in concurrence with the previous studies in Caucasians of British and Canadian origin. Comparison of the A and G alleles with HLA DR3–DR4 did not result in any significant P values. No significant association was found between SUMO4 M55V and LADA. SUMO4 M55V is not associated with susceptibility to T1DM and LADA in Latvians, and Latvians exhibit similarity to other Caucasians with respect to association of SUMO4 M55V with autoimmune diabetes.