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Time‐Resolved Fluorescence Imaging of Islet Cell Autoantibodies
Author(s) -
VUORINEN PAULI,
RULLI MARIS,
KUUSISTO ARI,
SIMELL SATU,
SIMELL TUULA,
VAHLBERG TERO,
ILONEN JORMA,
HYÖTY HEIKKI,
KNIP MIKAEL,
SIMELL OLLI
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1375.035
Subject(s) - fluorescence , autoantibody , bioanalysis , islet , serial dilution , chemistry , titer , fluorescence lifetime imaging microscopy , nuclear magnetic resonance , biomedical engineering , antibody , medicine , pathology , chromatography , optics , diabetes mellitus , immunology , endocrinology , physics , alternative medicine
Time‐resolved fluorescence of lanthanide chelates has been widely used in bioanalytical assays. Long fluorescence time, large Stokes shift, and minute fading out of the fluorescence over years are major advantages of the lanthanides over the conventional fluorescent dyes. We have now applied time‐resolved fluorescence imaging (TRFI) also for measurement of type 1 diabetes mellitus (T1DM)‐related islet cell autoantibodies (ICA). Retaining the accuracy of conventional ICA, TRFI has over 10 times better signal‐to‐noise ratio than the conventional fluorochromes. The technology allows objective determination of fluorescence intensity with the camera and computer software, and serial dilutions for obtaining the antibody titer in autoantibody‐positive samples are unnecessary.We now describe the TRFI as a method and its application for measurement of ICA.