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Pancreatic Autoimmunity Induction with Insulin B:9–23 Peptide and Viral Mimics in the NZB Mouse
Author(s) -
DEVENDRA DEVASENAN,
MIAO DONGMEI,
NAKAYAMA MAKI,
EISENBARTH GEORGE S.,
LIU EDWIN
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1375.020
Subject(s) - autoimmunity , insulitis , insulin , autoantibody , diabetes mellitus , peptide , immunology , medicine , endocrinology , biology , immune system , antibody , biochemistry
To create a new experimental model of diabetes, we used the New Zealand Black (NZB) mouse as a potential model. NZB mice were immunized with B:9–23 insulin peptide in IFA and the viral mimic, poly(A:U).No diabetes was observed but blood glucose was significantly higher in the B:9–23 peptide group compared to controls. Insulin autoantibodies (IAA) were only induced in groups given the B:9–23 peptide. B:9–23 alone induced peri‐insulitis. We demonstrate insulin autoimmunity in the NZB mouse using the insulin peptide B:9–23 and viral mimics. The reason for the protection from diabetes despite the presence of autoimmunity is currently not established.