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Evaluation of a Real‐Time PCR Assay to Detect Coxiella burnetii
Author(s) -
KLEE SILKE R.,
ELLERBROK HEINZ,
TYCZKA JUDITH,
FRANZ TATJANA,
APPEL BERND
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1374.111
Subject(s) - coxiella burnetii , q fever , real time polymerase chain reaction , biology , transposase , virology , genome , polymerase chain reaction , microbiology and biotechnology , genetics , gene , transposable element
We evaluated real‐time PCR assays for the detection of C. burnetii which targets sequences that are present either in one ( icd ) or in several copies (transposase of IS1111a ) on the chromosome. The assays are highly sensitive, with reproducible detection limits of approximately 10 copies per reaction, at least 100 times more sensitive than capture ELISA, when performed on infected placenta material and specific for C. burnetii . The numbers of IS1111 elements in the genomes of 75 C. burnetii isolates were quantified by real‐time PCR and proved to be highly variable.