Detection of  Coxiella burnetii  in Market Chicken Eggs and Mayonnaise | Zendy

TATSUMI NORIYUKI | Zendy; BAUMGARTNER ANDREAS | Zendy; QIAO YING | Zendy; YAMAMOTO IKKYU | Zendy; YAMAGUCHI KAZUO | Zendy

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Detection of Coxiella burnetii in Market Chicken Eggs and Mayonnaise

Author(s) -

TATSUMI NORIYUKI,

BAUMGARTNER ANDREAS,

QIAO YING,

YAMAMOTO IKKYU,

YAMAGUCHI KAZUO

Publication year - 2006

Publication title -

annals of the new york academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.712

H-Index - 248

eISSN - 1749-6632

pISSN - 0077-8923

DOI - 10.1196/annals.1374.096

Subject(s) - coxiella burnetii , q fever , biology , nested polymerase chain reaction , gene , virology , microbiology and biotechnology , polymerase chain reaction , genetics

We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii . The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 10 4 to 10 6 per egg, according to our quantitative PCR test.

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