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Detection of Coxiella burnetii in Market Chicken Eggs and Mayonnaise
Author(s) -
TATSUMI NORIYUKI,
BAUMGARTNER ANDREAS,
QIAO YING,
YAMAMOTO IKKYU,
YAMAGUCHI KAZUO
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1374.096
Subject(s) - coxiella burnetii , q fever , biology , nested polymerase chain reaction , gene , virology , microbiology and biotechnology , polymerase chain reaction , genetics
We tried to detect C. burnetii in market chicken eggs and mayonnaise by nested PCR assay. The PCR target was the com 1 gene of C. burnetii . The positive rate for egg and mayonnaise samples was 4.2% and 17.6%, respectively. Direct sequence of some of the positive egg samples shows mutations whereas no mutation was found in the positive mayonnaise samples. The number of molecules of the Q fever agent is estimated at 10 4 to 10 6 per egg, according to our quantitative PCR test.