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Detection of Rickettsia rickettsii and Rickettsia sp. in Blood Clots in 24 Patients from Different Municipalities of the State of São Paulo, Brazil
Author(s) -
GEHRKE FLÁVIA SOUSA,
MENDES do NASCIMENTO ELVIRA MARIA,
RODRIGUES de SOUZA ELIANA,
COLOMBO SILVIA,
JACINTHO da SILVA LUIZ,
SCHUMAKER TERESINHA TIZU SATO
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1374.047
Subject(s) - rickettsia rickettsii , rickettsia , polymerase chain reaction , biology , virology , gene , dna sequencing , microbiology and biotechnology , spotted fever , genetics , virus
The authors detected Rickettsia genus organisms using shell vial and polymerase chain reaction (PCR)/sequencing analysis in blood clots in patients suspected of having Brazilian spotted fever (BSF). DNA was detected using PCR with three sets of primers to access the glt A, omp A, and omp B genes. Sequence analysis was carried out using an automatic sequencer with Bioedit® software. Seventy‐five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. To date, 46% of the samples have been sequenced.