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Molecular Sequence Evidence for the Reclassification of Some Babesia Species
Author(s) -
ALLSOPP MARIA T. E. P.,
ALLSOPP BASIL A.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1373.076
Subject(s) - biology , babesia , theileria , phylogenetic tree , genetics , gene , ribosomal rna , 18s ribosomal rna , polymerase chain reaction , phylogenetics , ribosomal dna , genus , parasite hosting , zoology , virology , world wide web , computer science
Taxonomic characterization of organisms in the genera Theileria and Babesia was originally based on observations of morphology and certain general phenotypic characteristics, which enabled many parasites to be unequivocally assigned to a particular genus. However, application of molecular genetic techniques, such as the polymerase chain reaction (PCR) for gene amplification, and DNA sequencing, have revealed gross inconsistencies in the assignation of some parasite genetic variants, particularly those of the B. gibsoni and B. microti complexes, to the genus Babesia . These variants cannot be assigned, on the basis of sequence information and phylogenetic analysis, to either of the genera Theileria and Babesia . The gene for which most sequence information is available for phylogenetic analysis is the small subunit ribosomal RNA (srRNA) gene. This gene allows clear distinction of the genera Theileria and Babesia ( sensu stricto ) and reveals that many “ Babesia ” variants are phylogenetically distinct from both genera. This distinction is confirmed, for some of the variants, by β‐tubulin sequence data, suggesting that the organisms should be renamed and reclassified.