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Identification of Homologous Genes of T. annulata Proteins in the Genome of Theileria sp. (China)
Author(s) -
MIRANDA JOANA P.G.,
BAKHEIT MOHAMMED,
SCHNEIDER ILKA,
HALLER DANIEL,
AHMED JABBAR S.,
YIN HONG,
OLIVA ABEL G.,
SEITZER ULRIKE
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1373.067
Subject(s) - complementary dna , biology , gene , clone (java method) , microbiology and biotechnology , genome , genetics
Homologues to previously described Theileria (T.) annulata genes ( T. annulata surface protein [TaSP], putative T. annulata membrane protein [TaD]) were successfully amplified by polymerase chain reaction (PCR) from Theileria sp. (China) merozoite cDNA, with 88% identity to TaD; TcSP partial cDNA, 94% identity to TaSP. Moreover, homologues to a secretory protein of T. annulata (TaSE), with a sequence identity of 99% on the cDNA level (TcSE partial cDNA) and to a potential membrane protein of T. lestoquardi (Clone‐5), with a sequence identity of 100% on the genomic level (Tc Clone‐5) but lacking an intron at positions 1894–1928 were identified.