Premium
Evaluation of Cattle Inoculated with Babesia bovis Clones Adhesive In Vitro to Bovine Brain Endothelial Cells
Author(s) -
CANTO GERMINAL J.,
FIGUEROA JULIO V.,
RAMOS JUAN A.,
ROJAS EDMUNDO E.,
GARCIATAPIA DAVID,
ANTONIO ALVAREZ J.,
ALLRED DAVID R.,
CARSON CHARLES A.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1373.058
Subject(s) - babesia bovis , virulence , inoculation , biology , clone (java method) , feces , parasite hosting , babesiosis , virology , microbiology and biotechnology , group a , veterinary medicine , immunology , medicine , genetics , dna , world wide web , computer science , gene
A comparative assessment of the virulence of Babesia bovis clones that adhere or not to bovine brain endothelial cells was done using two clones of B. bovis : (1) a clone phenotypically characterized as virulent (2F8) and (2) a clone of reduced virulence (RAD). Of these subpopulations, we selected those that had adhesive characteristics (a) or nonadhesive characteristics (na) in cultured endothelial cells. Twenty Holstein cattle, 12 months of age or older, were used in this study, and these cattle were randomly assigned to five groups of four animals each. The clones and their respective subpopulations were inoculated via intramuscular injection at a 0.5 × 10 7 infected erythrocyte dosage. Group A was inoculated with aRAD, group B with naRAD, group C with a2F8, group D with na2F8, and group E remained as a control. All inoculated animals showed a decrease in the packed cell volume (PCV), with group D showing the largest decrease (39.53%) and longest time (7 days) with rectal temperature above 39.5°C. Babesia was observed in stained blood smears from only six cattle. While the four parasite subpopulations were pathogenic, significant differences were not noted among them, despite that the subpopulations considered to be virulent caused the greatest reduction in PCV per individual.