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Development of an Immunosensor for the Diagnosis of Bovine Anaplasmosis
Author(s) -
SILVA MARTA,
WILKOWSKY SILVINA,
DE ECHAIDE SUSANA TORIONI,
FARBER MARISA,
OLIVA ABEL
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1373.056
Subject(s) - anaplasmosis , antibody , bovine serum albumin , chemistry , recombinant dna , microbiology and biotechnology , virology , chromatography , biology , tick , immunology , biochemistry , gene
An optical immunosensor based in major surface protein 5 (MSP5) of Anaplasma marginale was developed towards detection of anti‐ Anaplasma sp. antibodies in acute infection as well as in vaccinated cattle. This study was performed using recombinant MSP5 covalently immobilised in controlled pore glass (CPG) beads to detect anti‐MSP5 antibodies in serum samples. The quantification is based on the measurement of the Cy5 fluorescence of the detection antibody, anti bovine IgG, after reaction with serum. Sera were collected in enzootic and tick‐free regions of Argentina. The immunosensor showed a detection range of 1.2 g/ml to 48 g/ml of antibody in sera, with a sensitivity of 93% and a specificity of 70%. The optical immunosensor developed is suitable for quantification of antibodies in sera of naturally or experimentally infected animals.