Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay | Zendy

GE JINYING | Zendy; WEN ZHIYUN | Zendy; WANG XIJUN | Zendy; HU SEN | Zendy; LIU YONGGANG | Zendy; KONG XIANGANG | Zendy; CHEN HUALAN | Zendy; BU ZHIGAO | Zendy

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Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay

Author(s) -

GE JINYING,

WEN ZHIYUN,

WANG XIJUN,

HU SEN,

LIU YONGGANG,

KONG XIANGANG,

CHEN HUALAN,

BU ZHIGAO

Publication year - 2006

Publication title -

annals of the new york academy of sciences

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.712

H-Index - 248

eISSN - 1749-6632

pISSN - 0077-8923

DOI - 10.1196/annals.1373.030

Subject(s) - vesicular stomatitis virus , virology , coronavirus , vesicular stomatitis indiana virus , glycoprotein , neutralization , rhabdoviridae , respiratory system , viral envelope , virus , medicine , biology , covid-19 , microbiology and biotechnology , pathology , anatomy , disease , infectious disease (medical specialty)

We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS‐CoV in trans . The neutralization and infection blocking tests showed that the VSV Δ G*SG and SARS‐CoV reacted similarly to SARS‐CoV specific antiserum, suggesting the VSVΔ G*SG can be a safe replacement of the live SARS‐CoV for neutralization test and cell‐entry assay.

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