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Generating Vesicular Stomatitis Virus Pseudotype Bearing the Severe Acute Respiratory Syndrome Coronavirus Spike Envelope Glycoprotein for Rapid and Safe Neutralization Test or Cell‐Entry Assay
Author(s) -
GE JINYING,
WEN ZHIYUN,
WANG XIJUN,
HU SEN,
LIU YONGGANG,
KONG XIANGANG,
CHEN HUALAN,
BU ZHIGAO
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1373.030
Subject(s) - vesicular stomatitis virus , virology , coronavirus , vesicular stomatitis indiana virus , glycoprotein , neutralization , rhabdoviridae , respiratory system , viral envelope , virus , medicine , biology , covid-19 , microbiology and biotechnology , pathology , anatomy , disease , infectious disease (medical specialty)
We generated a recombinant vesicular stomatitis virus (VSV) pseudotype (VSV Δ G*SG) by replacing the envelope G gene with the GFP gene and complementing with spike glycoprotein (S) of SARS‐CoV in trans . The neutralization and infection blocking tests showed that the VSV Δ G*SG and SARS‐CoV reacted similarly to SARS‐CoV specific antiserum, suggesting the VSVΔ G*SG can be a safe replacement of the live SARS‐CoV for neutralization test and cell‐entry assay.