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Genotyping of Newcastle Disease Viruses Isolated from 2002 to 2004 in China
Author(s) -
WANG ZHILIANG,
LIU HUALEI,
XU JIANGTAO,
BAO JINGYUE,
ZHENG DONGXIA,
SUN CHENGYING,
WEI RONG,
SONG CUIPING,
CHEN JIMING
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1373.027
Subject(s) - newcastle disease , phylogenetic tree , genotype , biology , virology , virulence , outbreak , genotyping , virus , genetics , strain (injury) , gene , anatomy
The main function region of the fusion (F) protein gene of 124 strains of Newcastle disease virus isolated from 2002 to 2004 in China was amplified and sequenced for further phylogenetic and residue substitutive analysis. Most of the isolates were classified into genotype VIIc, VIId, VIf, and VIb, while others into genotype IX, III, or II. The genotype IX, a unique genotype which includes strain F48, the first Chinese virulent NDV strain isolated in 1948, were still found inducing sporadic infections in certain areas. Subgenotype VIIc, VIId, and VIIe viruses, which were distributed in clusters in the phylogenetic tree distinct from members of subgenotypes VIIa and VIIb, were responsible for most outbreaks in China and circulated predominantly in China in recent years. Strain NDV03‐026, an isolate of the genotype II which was normally lentogenic, was found carrying 112 RRQKRF 117 motif at the cleavage site of F protein as the virulent strain.