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The Effect of a Fat‐Enriched Diet on the Pattern of Distribution of Pancreatic Islet Cells in the C57BL/6J Mice
Author(s) -
ADEGHATE ERNEST,
CHRISTOPHER HOWARTH FRANK,
RASHED HAMEED,
SAEED TARIQ,
GBEWONYO AMSTRONG
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1372.002
Subject(s) - medicine , endocrinology , islet , hyperinsulinemia , pancreatic islets , glucagon , somatostatin , insulin , pancreas , pancreatic polypeptide , biology , chemistry , insulin resistance
Abstract : The C57BL/6J mice are inbred strains and develop the metabolic syndrome of obesity, hyperinsulinemia, hyperglycemia, and hypertension, when fed a high‐fat diet. These features are similar to those observed in the human metabolic syndrome. This article examined the effect of fat‐enriched (FE) diet on the pattern of distribution of insulin‐, glucagon‐, somatostatin‐, and pancreatic polypeptide (PP)‐positive cells in the pancreatic islets of C57BL/6J mice using immunohistochemical methods. Insulin‐immunoreactive cells were observed in both the peripheral and central regions of the islets of Langerhans in both FE‐ and control diet‐fed mice. The percentage distribution of insulin‐positive cells was similar in FE (83.5 ± 6.4) compared to control diet‐fed C57BL/6J mice (83.8 ± 6.5). Glucagon‐containing cells were discerned in the periphery of pancreatic islets in both FE‐ and control diet‐fed mice. The percentage distribution of glucagon was not statistically different in mice fed with FE (9.9 ± 2.7) compared to control diet (11.3 ± 4.9). Somatostatin‐positive cells were seen in the outer part of the islet of Langerhans and constitute 12.1% (±6.3) and 10% (±5.5) of pancreatic islet cells in FE‐ and control diet‐fed mice, respectively. PP‐immunoreactive cells were observed in the peripheral region of the pancreatic islets of both FE‐ and control diet‐fed mice. The percentage distribution of PP‐positive cells was significantly (2.0 ± 1.2) lower compared to control (5.1 ± 2.4). In conclusion, the number of PP is significantly reduced in FE diet‐fed mice and may play a role in the pathogenesis of diet‐induced metabolic syndrome in C57BL/6J mice.