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Inclusion Dynamics in PC12 is Comparable Between Amphetamines and MPTP
Author(s) -
GESI MARCO,
LAZZERI GLORIA,
FERRUCCI MICHELA,
PELLEGRINI ANTONIO,
LENZI PAOLA,
RUGGIERI STEFANO,
FORNAI FRANCESCO,
PAPARELLI ANTONIO
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1369.028
Subject(s) - mptp , neurotoxin , chemistry , methamphetamine , amphetamine , metabolite , catecholamine , intracellular , biophysics , neurotoxicity , dopamine , pharmacology , toxicity , biochemistry , endocrinology , biology , dopaminergic , organic chemistry
In previous studies it was demonstrated that amphetamine derivatives and 1‐methyl article‐4‐phenylpyridinium produce neuronal cell bodies. In the present work, we compared the fine ultrastructure of the intracellular inclusions induced by these different neurotoxic treatments. In particular, we compared the dynamical changes occurring when a mild toxic stimulus acts for different time intervals. For this purpose, we exposed catecholamine‐synthesizing PC12 cells to different amphetamine derivatives (methamphetamine and 3,4‐methylenedioxymethamphetamine), or 1‐methyl‐4‐phenylpyridinium ion, which represents the active metabolite of the neurotoxin 1‐methyl‐4‐phenyl‐1,3,4,6‐tetrahydropyridine. Despite inclusions that are elicited by different mechanisms depending on the specific neurotoxin, their ultrastructural features are similar and there is a high parallelism in their temporal evolution. This suggests that formation of inclusions is a multi‐step process that might be elicited by different stimuli and, once triggered, leads to the same final effect.