Premium
Isolation and Comparative Study of Cell‐Free Nucleic Acids from Human Urine
Author(s) -
BRYZGUNOVA OLGA E.,
SKVORTSOVA TATYANA E.,
KOLESNIKOVA ELENA V.,
STARIKOV ANDREY V.,
RYKOVA ELENA YU,
VLASSOV VALENTIN V.,
LAKTIONOV PAVEL P.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1368.045
Subject(s) - urine , nucleic acid , dna , cell free fetal dna , microbiology and biotechnology , dna methylation , chemistry , cell , rna , gene , biochemistry , biology , gene expression , genetics , fetus , pregnancy , prenatal diagnosis
Abstract: Cell‐free nucleic acids (NA) from human urine were investigated. Concentrations of DNA and RNA in the urine of healthy people were independent of gender and were in the range of 6 ng/mL to 50 ng/mL and 24 ng/mL to 140 ng/mL, respectively. DNA fragments of 150–400 bp represent the main part of cell‐free DNA, along with DNA fragments up to 1,300 bp, which were found in male urine, and DNA fragments up to 19 kbp, which were found in female urine. Analysis of circulating DNA, isolated from blood of breast cancer patients and cell‐free DNA isolated from their urine by methylation‐specific PCR, demonstrates that the presence of methylated promoters of RASSF1A and RARβ2 genes in plasma was accompanied by the detection of the same methylated markers in urine. The data obtained demonstrate applicability of cell‐free urine DNA in cancer diagnostics.