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MALDI‐TOF Mass Spectrometry for Quantitative, Specific, and Sensitive Analysis of DNA and RNA
Author(s) -
DING CHUNMING,
LO YUK MING DENNIS
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1368.038
Subject(s) - rna , dna , microbiology and biotechnology , cell free fetal dna , cell , chemistry , fetus , biology , biochemistry , pregnancy , prenatal diagnosis , gene , genetics
Cell‐free fetal DNA and RNA released into the maternal circulation offer new opportunities to study fetal and pregnancy‐associated abnormalities. Similarly, tumor cells can release cell‐free DNA and RNA into the peripheral circulation, and these cell‐free DNA and RNA can be used for cancer diagnosis, monitoring, and prognosis. However, these DNA and RNA often exist at very low concentrations (for fetal DNA, ∼20 genome‐equivalents (G.E.)/mL of plasma in the first trimester). The analysis is further complicated by the predominant amount of blood cell‐derived DNA and RNA. MALDI‐TOF mass spectrometry can provide quantitative, specific, and sensitive analysis of DNA and RNA, and thus may be a useful technology for the field.