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A Surfactant Copolymer Facilitates Functional Recovery of Heat‐Denatured Lysozyme
Author(s) -
WALSH ALEXANDRA M.,
MUSTAFI DEVKUMAR,
MAKINEN MARVIN W.,
LEE RAPHAEL C.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1363.029
Subject(s) - lysozyme , poloxamer , copolymer , pulmonary surfactant , chemistry , chromatography , nonionic surfactant , enzyme , biochemistry , organic chemistry , polymer
A bstract : The triblock copolymer poloxamer 188 is a non‐cytotoxic, nonionic surfactant with both hydrophobic and hydrophilic domains. We show that P188 is able to facilitate the recovery of catalytic activity of heat‐denatured lysozyme in dilute solution at low molar ratios of P188:enzyme. Heat‐denatured enzyme retained 55% of native activity. After treatment with P188, the enzyme's activity was 85% of native. Because of the low molar ratios used and the non‐cytotoxic nature of the compound, P188 may be of potential use in burn therapy.