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Phylogenetic Analysis of a Novel Molecular Isolate of Spotted Fever Group Rickettsiae from Northern Peru: Candidatus Rickettsia andeanae
Author(s) -
JIANG JU,
BLAIR PATRICK J.,
FELICES VIDAL,
MORON CECILIA,
CESPEDES MANUEL,
ANAYA ELIZABETH,
SCHOELER GEORGE B.,
SUMNER JOHN W.,
OLSON JAMES G.,
RICHARDS ALLEN L.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1355.054
Subject(s) - biology , spotted fever , rickettsia , phylogenetic tree , candidatus , rickettsiaceae , polymerase chain reaction , virology , gene , genetics , 16s ribosomal rna , virus
A bstract : Phylogenetic analysis of five rickettsial genes (17‐kDa gene, gltA, ompB, ompA , and sca4 ) from two molecular isolates of Candidatus Rickettsia andeanae from two ticks ( Amblyomma maculatum and Ixodes boliviensis ) collected from two domestic horses living in two separate locations in northern Peru (Coletas and Naranjo) was conducted to more clearly characterize this recently reported novel spotted fever group (SFG) rickettsia. Following nested polymerase chain reaction (PCR) amplification of the17‐kDa gene, gltA, ompB, ompA , and sca4 , amplicons were purified, sequenced, and compared to those downloaded from GenBank. Phylogenetic analyses of the Candidatus Rickettsia andeanae sequences generated from17‐kDa gene (483 bp), gltA (1185 bp), ompA (1598 bp), ompB (4839 bp), and sca4 (2634 bp) demonstrated that they aligned strongly with those of SFG rickettsiae. Moreover, the sequences of these five genes most closely aligned with the following rickettsiae: ompA : Rickettsia sp RpA4 (98.03%), R. sp DnS28 (97.90%), and R. rhipicephali and R. massiliae (97.11%); ompB : R. aeschlimannii (97.22%), R. rhipicephali (97.20%), and R. sp Bar 29 (97.10%); and sca4 : R. massiliae (97.8%), R. rhipicephali , and R. slovaca (97.7%). These results from the additional phylogenetic analyses of Candidatus Rickettsia andeanae confirm its inclusion within, and distance and uniqueness from, other known SFG rickettsiae.