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Analysis of Immunoprotectivity of the Recombinant OmpA of Rickettsia heilongjiangensis
Author(s) -
JIAO YANMEI,
WEN BOHAI,
CHEN MEILING,
NIU DONGSHENG,
ZHANG JUN,
QIU LING
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1355.042
Subject(s) - spotted fever , biology , recombinant dna , heterologous , rickettsia , virology , rickettsia rickettsii , antibody , microbiology and biotechnology , immune system , gene , virus , immunology , biochemistry
A bstract : A truncated gene ompA was amplified from Rickettsia heilongjiangensis isolated in China and a 56‐kDa truncated OmpA protein was expressed in E. coli cells transformed with the ompA ‐recombined expression plasmid. High levels of serum antibodies to R. heilongjiangensis and proliferation of the splenic cells were found in mice immunized with the truncated OmpA. After challenge with R. heilongjiangensis or R. rickettsii , fever and pathological damages of the guinea pigs immunized with the truncated OmpA were significantly slighter as compared with those of nonimmunized guinea pigs. These results suggest that the truncated OmpA of R. heilongjiangii is immunogenic for effectively inducing humoral and cell‐mediated immune responses against homologous and heterologous species in the spotted fever group.