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Balb/c Mouse Model and Real‐Time Quantitative Polymerase Chain Reaction for Evaluation of the Immunoprotectivity against Q Fever
Author(s) -
ZHANG JINGBO,
WEN BOHAI,
CHEN MEILING,
ZHANG JUN,
NIU DONGSHENG
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1355.027
Subject(s) - coxiella burnetii , q fever , immunogen , real time polymerase chain reaction , spleen , biology , virology , immunization , polymerase chain reaction , microbiology and biotechnology , antigen , immunology , antibody , monoclonal antibody , biochemistry , gene
A bstract : The Balb/c mice were infected with Coxiella burnetii and samples of blood and major organs from the infected mice were collected on days 1 to 14 after infection. The DNAs extracted from the samples were detected by a developed real‐time quantitative PCR specific for C. burnetii and high loads of C. burnetii were found in spleens, livers, and lungs, particularly in spleens. The Balb/c mice were immunized with whole cell antigen (WCA) of C. burnetii and coxiella loads in spleens of mice were assessed by the real‐time quantitative PCR on day 7 after challenge with C. burnetii . The analysis suggested that phase I whole cells were excellent immunogen that elicited complete protection against coxiella infection by two‐booster but not one‐booster immunization. The results suggest that the combination of Balb/c model and the real‐time quantitative PCR assay is a reliable and sensitive way to evaluate the efficiency of vaccines against Q fever.