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Phylogenic Analysis of Rickettsial Patatin‐like Protein with Conserved Phospholipase A 2 Active Sites
Author(s) -
BLANC GUILLAUME,
RENESTO PATRICIA,
RAOULT DIDIER
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1355.012
Subject(s) - biology , gene , gene duplication , phylogenetics , genome , phylogenetic tree , genetics , rickettsia , phospholipase a2 , phospholipase a , microbiology and biotechnology , biochemistry , enzyme , virus
A bstract : Genome analysis of Rickettsia felis highlighted the presence of three patatin‐like protein (PLP) genes ( pat1 , pat2A , and pat2B ), whereas only one PLP gene ( pat1 ) is found in the other sequenced rickettsial genomes. Here, we aligned the rickettsial PLPs with characterized patatins from plants and found that they possess all the conserved amino acid residues identified as important for phospholipase A 2 activity. We also carried out a phylogenic analysis of the rickettsial PLPs together with bacterial and eukaryotic homologs. The phylogeny of the rickettsial Pat1 proteins is in conflict with the currently recognized Rickettsia phylogeny. Possible scenarios that might explain this incongruence are discussed and involve either gene conversion or gene duplication events.