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Identification, Cloning, and Expression of Potential Diagnostic Markers for Q Fever
Author(s) -
CHAO C C,
CHEN H W,
LI X,
XU W B,
HANSON B,
CHING W M
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1355.010
Subject(s) - antigen , coxiella burnetii , cloning (programming) , vector (molecular biology) , serology , virology , biology , expression cloning , computational biology , immunology , antibody , gene , genetics , recombinant dna , computer science , peptide sequence , programming language
A bstract : The clinical diagnosis of Q fever is difficult. Whole cell antigens are currently used in several serological methods, but antigens are limited due to the hazardous nature of Coxiella burnetii cultivation. In this report, we described the method of detecting immunodominant antigens of C. burnetii by using proteomic techniques with patient sera, and cloning and expressing the selected antigens using a novel vector known for its ease of expression, purification, and downstream application.