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Dissecting the Rickettsia prowazekii Genome: Genetic and Proteomic Approaches
Author(s) -
TUCKER AIMEE M.,
PANNELL LEWIS K.,
WOOD DAVID O.
Publication year - 2005
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1355.005
Subject(s) - rickettsia prowazekii , proteome , biology , gene , obligate , rickettsia , genome , proteomics , computational biology , genetics , ecology , virus
A bstract : The obligate nature of Rickettsia prowazekii intracellular growth places severe restrictions on the analysis of rickettsial gene function and gene expression. Fortunately, this situation is improving as methods for the genetic manipulation and proteomic analysis of this fascinating human pathogen become available. In this paper, we review the current status of rickettsial genetics and the isolation of rickettsial mutants using a genetic approach. In addition, the examination of rickettsial gene expression through characterization of the rickettsial proteome will be described. This will include a description of a high‐throughput, accurate mass approach that has identified 596 rickettsial proteins in a complex rickettsial protein sample.