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MAP Kinases and Heat Shock–Induced Hormesis in Human Fibroblasts during Serial Passaging in Vitro
Author(s) -
NIELSEN ELISE R.,
ESKILDSENHELMOND YVONNE E.G.,
RATTAN SURESH I.S.
Publication year - 2006
Publication title -
annals of the new york academy of sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.712
H-Index - 248
eISSN - 1749-6632
pISSN - 0077-8923
DOI - 10.1196/annals.1354.048
Subject(s) - longevity , p38 mitogen activated protein kinases , hormesis , kinase , in vitro , mitogen activated protein kinase , microbiology and biotechnology , biology , basal (medicine) , heat shock protein , andrology , medicine , biochemistry , protein kinase a , endocrinology , genetics , oxidative stress , gene , insulin
Adult human skin fibroblasts were exposed repeatedly to 41°C or 42°C heat shock (HS) for 1 h twice a week during serial passaging throughout their replicative life span. On the basis of longevity curves, cell size, and morphology, we observed that repeated mild heat shock (RMHS) at 41,°C had strong anti‐aging hormetic effects, including 20% extension of cellular longevity. The basal levels of the MAP kinases JNK1, JNK2, and p38 increased during serial passaging, while that of ERK2 decreased. RMHS further exaggerated these effects, which suggests that age‐related changes in MAP kinases may be an adaptive response for better cell survival.